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Morning glory Ipomoea nil (=Pharbitis nil)

Tokyo Kokei Standard Decotylated embryo culture --> Callus and various forms of protuberances

Copyright 1998-2017 Yoshiaki Yoneda

After removing the cotyledons surgically from the embryo of a cotyledon stage one week after fertilization and culturing a hypocotyl including a radicle, a callus occurred from a cut end, and projections of various shapes (tubes, cups, bars) arose from the border region of a callus and a hypocotyl. With this technique, you don't need to add any particular hormone to agar nutrient medium.

Slide No. 252

1 x 1

4 x 4

2 x 2

lens 8 x 8

16 x 16

1 x 1 (thumbnail images) : For indexing
2 x 2, 4 x 4, 8 x 8 : For viewing on monitor
8 x 8, 16 x 16 : For printing

Edited by Yuuji Tsukii (Lab. Biology, Science Research Center, Hosei University)