Copyright 1998-2022 Yoshiaki Yoneda
After removing the cotyledons surgically from the embryo of a cotyledon stage one week after fertilization and culturing a hypocotyl including a radicle, a callus occurred from a cut end, and projections of various shapes (tubes, cups, bars) arose from the border region of a callus and a hypocotyl.
With this technique, you don't need to add any particular hormone to agar nutrient medium.
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